カシマタ　マサノリ   KASHIMATA MASANORI   柏俣　正典    所属   朝日大学  歯学部 基礎教育系 英語    職種   教授
言語種別	英語
発行・発表の年月	2022/03
形態種別	研究論文
査読	査読あり
標題	Retinal damage alters gene expression profile in lacrimal glands of mice
執筆形態	共著
掲載誌名	Journal of Pharmacological Sciences
掲載区分	国外
巻・号・頁	149(1),pp.20-26
総ページ数	7
担当範囲	研究総括
担当区分	最終著者
概要	We hypothesized that retinal damage might alter gene expression in the lacrimal gland, leading to those changes in tear components. We caused retinal damage in mice by intravitreal injection of N-methyl-d-aspartate (NMDA) or excessive light exposure. Hematoxylin and eosin staining showed no histological changes in the lacrimal glands of animals whose retinas had been damaged. However, RNA sequencing of lacrimal glands on the 3rd day after NMDA injection or light exposure revealed changes in the expression of 491 genes (268 up-regulated; 223 down-regulated) in the NMDA group and 531 genes (311 up-regulated; 220 down-regulated) in the light group. Further gene-set enrichment analysis indicated that both types of retinal damage activated the immune system in the lacrimal glands. This is the first demonstration that retinal damage can alter gene expression in the lacrimal glands, and it might lead to a novel non-invasive screening method for early detection of retinal diseases.