トモフジ　タカアキ   TOMOFUJI TAKAAKI   友藤　孝明    所属   朝日大学  歯学部 口腔感染医療学講座 社会口腔保健学    朝日大学  大学院 歯学研究科    職種   教授
言語種別	英語
発行・発表の年月	2017/12
形態種別	研究論文（学術雑誌）
標題	Age-related changes of CD4+ T cell migration and cytokine expression in germ-free and SPF mice periodontium.
執筆形態	共著
掲載誌名	Arch Oral Biol
掲載区分	国外
巻・号・頁	87(5),pp.72-78
著者・共著者	Tomofuji T
概要	Increasing age is a potential risk factor for periodontal tissue breakdown, which may be affected by commensal flora. The aim of this study evaluated age-related changes in CD4+ T cells, C-C chemokine ligand 5 (CCL5), interleukin (IL)-17A, and receptor activator of nuclear factor-kappa B ligand (RANKL) expression using germ-free (GF) and conventionally reared (SPF) mice. SPF mice, but not GF mice, showed an age-related increase in alveolar bone loss (P < 0.01). SPF mice at 22 weeks of age increased expression of CD4+ T cells, CCL5, IL-17A, and RANKL compared to those at 8 weeks of age in connective tissue and alveolar bone surface (P < 0.01). Furthermore, there was increased CD4+ T cells, which were co-expressed with IL-17A and RANKL in SPF mice at 22 weeks of age. On the other hand, the GF mice did not show any significant differences in CD4+ T cells, CCL5, IL-17A and RANKL expression between the two age groups.
ISSN	0003-9969
PMID	28528308