タキガワ　トシヤ   TAKIGAWA TOSHIYA   滝川　俊也    所属   朝日大学  歯学部 口腔構造機能発育学講座 口腔解剖学（口腔解剖学）    朝日大学  大学院 歯学研究科    職種   教授
言語種別	英語
発行・発表の年月	2020/09
形態種別	研究論文
査読	査読あり
標題	Genetic background influences the capacity for medial edge epithelium disintegration and phenotype of cleft palate in TGFβ3 knockout mice
執筆形態	共著
掲載誌名	Journal of Oral Biosciences
掲載区分	国外
出版社・発行元	ELSEVIER
巻・号・頁	Volume 62(Issue 3),pp.260-266
総ページ数	6
担当範囲	Second author and Corresponding author
著者・共著者	Sugiyama Akiko, Takigawa Toshiya
概要	We produced TGFβ3 knockout congenic mouse strains with five different genetic backgrounds. The phenotypes of the congenic strains were determined by visual examination. The capacity for disintegration of the medial edge epithelium (MEE) and basement membrane (BM) of palatal shelves of all five mouse strains was analyzed by using immunofluorescence staining after single palatal shelf suspension culture. Although the five congenic strains carried the same defective Tgfb3 gene, the fetal palate phenotypes differed among strains. The loss of the medial edge epithelial (MEE) cells and the basement menbrane (BM) of palate also differed with the genetic background, and the degree of such loss correlated with the cleft palate phenotype. Thus the cleft palate phenotype in mice is influenced by the genetic background, which governs the capacity for MEE and BM disintegration.